TREHALOSE AND A CALCIUM CHELATOR FOR RAM SEMEN CRYOPRESERVATION
نویسندگان
چکیده
منابع مشابه
Comparison of different diluents based on liposomes and egg yolk for ram semen cooling and cryopreservation
Background: Sperm cryopreservation is an important tool for breed improvement, nonetheless, spermatozoids of rams are extremely sensitive to cryopreservation. Aims: The present research was to compare a liposome-based (OptiXcell: OX) diluent, a commercial TRIS-egg yolk (Optidyl: OP) and a citrate egg yolk-based (CY) diluent on ovine semen quality through the cr...
متن کاملDoes natural honey act as an alternative to antibiotics in the semen extender for cryopreservation of crossbred ram semen?
Antibiotics are added to semen extenders to take care of heavy microbial load, however, their continuous use poses a constant threat of developing antibiotic resistance by the common microbes present in the semen. Our hypothesis was that natural honey, having antibacterial activity and rich in fructose could replace the use of antibiotics and fructose in the semen extender. Twenty-four ejaculat...
متن کاملIn Vitro Comparison of Soybean Lecithin Based-Extender with Commercially Available Extender for Ram Semen Cryopreservation
Background Egg yolk is the main cryoprotectant agent in semen freezing extenders which is used in order to protect spermatozoa against cold shock. However elimination of animal bioproducts from the cryopreservation protocol is becoming mandatory. Therefore the aim of this study is to compare a previously studied homemade soya bean lecithin based extender with a commercially available extender f...
متن کاملP-127: In Vitro Assessment of Trehalose for Cryopreservation of Ram Sperm in Extender Containing Soybean Lecithin
Background: Trehalose, a non-permeable disaccharide, has a protective role related both to osmotic effect and specific interactions with membrane phospholipids, rendering hypertonic media, causing cellular osmotic dehydration before freezing, and then decreasing the amount of cell injury by ice crystallization. Within this study, an attempt was made to evaluation of two levels of 50 and 100 mM ...
متن کاملIn Vitro Comparison of Soybean Lecithin Based-Extender with Commercially Available Extender for Ram Semen Cryopreservation
Background: Egg yolk is the main cryoprotectant agent in semen freezing extenders which is used in order to protect spermatozoa against cold shock. However, elimination of animal bioproducts from the cryopreservation protocol is becoming mandatory. Therefore, the aim of this study is to compare a previously studied, homemade soya bean lecithin based extender with a commercially available extend...
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ژورنال
عنوان ژورنال: Archives of Veterinary Science
سال: 2014
ISSN: 1517-784X
DOI: 10.5380/avs.v19i2.31064